INDUCIBLE MOSAICISM

Smart overview of the Invention

Inducible mosaicism focuses on creating multiple unique genetic edits in plant seeds. The method involves introducing a nucleic acid that encodes a DNA modification enzyme and potentially a guide RNA into plant cells or tissues. An inducible system is activated at specific growth stages, facilitating targeted expression of the DNA modification enzyme in key reproductive cells, leading to diverse genetic edits in the resulting seeds.

Field of Application

This technology pertains to plant biotechnology, particularly gene editing aimed at enhancing crop traits. The approach allows for precise modifications during predetermined stages of plant development, addressing challenges faced in traditional gene editing methods.

Importance of Gene Editing

Gene editing is vital for improving crop yield and quality, but achieving specific edits can be labor-intensive and inefficient. Current methods like CRISPR and TALEN have limitations, particularly in obtaining diverse alleles or large deletions. The proposed system seeks to overcome these hurdles by enabling efficient heritable edits and reducing the time and cost associated with generating genetic diversity.

Methodology Details

The described method includes introducing an expression cassette containing a DNA modification enzyme, optional guide RNA, and an inducible factor. Various chemicals can trigger this inducible factor at desired plant development stages. Different systems can be employed, such as alcohol-dependent or lactose-dependent effectors, to regulate the editing process effectively.

Potential Outcomes and Applications

The final product is a plant with progeny exhibiting multiple unique genetic edits, including indel mutations or allele replacements. This method can be applied to various dicotyledonous plants like Arabidopsis, soybean, and tomato. By streamlining the gene editing process, this technology could significantly enhance agricultural productivity and sustainability.