METHOD FOR ANCHORING AND MODIFYING NANO-DRUG ON SURFACE OF LIVING CELL

Smart overview of the Invention

The patent application introduces a method for anchoring and modifying nano-drugs on the surface of living cells. This involves introducing an active reactive group to the cell surface using a hydrophobic tail chain of a cell membrane anchoring molecule. The nano-drug is modified with a corresponding reactive group, and a biological orthogonal click reaction is executed between these groups to successfully anchor the nano-drug to the cell surface. This method offers a novel platform for cell modification, enhancing treatment efficacy compared to traditional methods.

Technical Field

This innovation falls within the biotechnology sector, specifically focusing on methods for anchoring and modifying nano-drugs on living cell surfaces. It addresses limitations in current nano-drug delivery systems by utilizing endogenous cells for improved targeting efficiency and therapeutic outcomes.

Background

Nano-drugs have been widely used since their inception in 1964, but they face challenges in reaching target sites due to physiological barriers, resulting in low targeting efficiency. Endogenous cells have been explored as delivery tools to enhance nano-drug targeting and efficacy. Current methods for cell surface modification include chemical reactions, glycosylation, genetic engineering, and physical methods, all of which have limitations such as complexity, time consumption, and potential interference with cellular functions.

Objectives and Methodology

The invention aims to overcome existing method limitations by providing a new approach to anchor and modify nano-drugs on cell surfaces. The process involves introducing an active reactive group via a cell membrane anchoring molecule's hydrophobic tail chain, modifying the nano-drug with a corresponding reactive group, and performing a biological orthogonal click reaction to achieve successful cell surface modification.

Applications and Advantages

This method holds potential for wide applications in treating various diseases by creating living cells modified with nano-drugs. It utilizes primary or immortalized human or animal cells, including T cells and neutrophils. The method supports the use of diverse therapeutic agents like hydrophobic drugs, hydrophilic drugs, protein therapeutics, and gene therapies. By improving targeting efficiency and therapeutic effects, this innovation represents a significant advancement in drug delivery systems.