ANIMAL FREE- ALPHA-LACTALBUMIN AND BETA-LACTOGLOBULIN FUSION MILK PROTEINS AND A PROCESS FOR PREPARING THE SAME

Smart overview of the Invention

The invention pertains to the field of biotechnology, focusing on animal-free fusion milk proteins and their production in recombinant host cells. It introduces vectors and expression cassettes for expressing these proteins in host cells, offering an alternative to traditional dairy products. The invention aims to address environmental and health concerns associated with conventional milk production.

Background and Need

With over 7.5 billion consumers globally, the demand for milk is rising, especially in developing countries. Traditional dairy farming contributes significantly to greenhouse gas emissions, impacting the environment and human health. Antibiotic use in animal farming leads to resistant microorganisms, posing a public health risk. The invention seeks to provide sustainable alternatives through cellular agriculture, reducing reliance on animal farming.

Summary of the Invention

This invention introduces a process for producing animal-free fusion proteins in recombinant host cells. It includes a recombinant vector system with polynucleotide sequences encoding these proteins. Additionally, methods for purifying and isolating the target proteins are provided, ensuring the production of safe and identical alternatives to natural bovine milk proteins.

Objectives

• Develop animal-free fusion proteins mimicking natural milk proteins from Bos indicus.
• Create methods for producing these proteins in recombinant host cells.
• Design recombinant vectors with sequences encoding fusion milk proteins.
• Produce compositions containing these proteins with acceptable carriers.
• Provide food compositions incorporating these fusion milk proteins.

Detailed Description and Definitions

The invention provides an exemplary embodiment of creating fusion proteins through genetic engineering. It defines key terms such as "milk protein," "animal-free milk proteins," and "fusion protein." The description emphasizes the innovative approach in producing milk proteins without involving mammals, using genetically engineered organisms to achieve this goal.